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Diurnal preference predicts phase differences in expression of human peripheral circadian clock genes

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dc.contributor.authorFerrante, Andrew
dc.contributor.authorGellerman, David
dc.contributor.authorAy, Ahmet
dc.contributor.authorWoods, Kerri
dc.contributor.authorFilipowicz, Allan
dc.contributor.authorBearden, Neil
dc.contributor.authorIngram, Kristian Kenyon
dc.contributor.authorJain, Kriti
dc.contributor.rorhttps://ror.org/02jjdwm75
dc.date.accessioned2024-07-08T13:14:56Z
dc.date.available2024-07-08T13:14:56Z
dc.date.issued2015
dc.description.abstractBackground: Circadian rhythms play an integral role in human behavior,physiology and health. Individual differences in daily rhythms (chronotypes) can affect individual sleep-wake cycles,activity patterns and behavioral choices. Diurnal preference,the tendency towards morningness or eveningness among individuals,has been associated with interpersonal variation in circadian clock-related output measures,including body temperature,melatonin levels and clock gene mRNA in blood,oral mucosa,and dermal fibroblast cell cultures. Methods: Here we report gene expression data from two principal clock genes sampled from hair follicle cells,a peripheral circadian clock. Hair follicle cells from fourteen individuals of extreme morning or evening chronotype were sampled at three time points. RNA was extracted and quantitative PCR assays were used to measure mRNA expression patterns of two clock genes,Per3 and Nr1d2. Results: We found significant differences in clock gene expression over time between chronotype groups,independent of gender or age of participants. Extreme evening chronotypes have a delay in phase of circadian clock gene oscillation relative to extreme morning types. Variation in the molecular clockwork of chronotype groups represents nearly three-hour phase differences (Per3: 2.61 hours; Nr1d2: 3.08 hours,both: 2.86) in circadian oscillations of these clock genes. Conclusions: The measurement of gene expression from hair follicles at three time points allows for a direct,efficient method of estimating phase shifts of a peripheral circadian clock in real-life conditions. The robust phase differences in temporal expression of clock genes associated with diurnal preferences provide the framework for further studies of the molecular mechanisms and gene-by-environment interactions underlying chronotype-specific behavioral phenomena,including social jetlag. © 2015 The Author(s).
dc.description.keywordChronobiology
dc.description.keywordChronotype
dc.description.keywordCircadian clock
dc.description.keywordGene expression
dc.formatapplication/pdf
dc.identifier.citationFerrante, A., Gellerman, D., Ay, A., Woods, K. P., Filipowicz, A. M., Jain, K., ... & Ingram, K. K. (2015). Diurnal preference predicts phase differences in expression of human peripheral circadian clock genes. Journal of circadian rhythms, 13.
dc.identifier.doihttps://doi.org/10.5334/jcr.ae
dc.identifier.issn17403391
dc.identifier.officialurlhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84989333092&doi=10.5334%2fjcr.ae&partnerID=40&md5=7badbcf02b3ae9d68626034829b32309
dc.identifier.urihttps://hdl.handle.net/20.500.14417/3097
dc.issue.number1
dc.journal.titleJournal of Circadian Rhythms
dc.language.isoeng
dc.page.final7
dc.page.initial1
dc.page.total40
dc.publisherBioMed Central Ltd.
dc.relation.departmentHuman Resources & Organisational Behaviour
dc.relation.entityIE University
dc.relation.schoolIE Business School
dc.rightsAttribution 4,0 International
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectChronobiology; Chronotype; Circadian clock; Gene expression
dc.subject.keywordChronobiology
dc.subject.keywordChronotype
dc.subject.keywordCircadian clock
dc.subject.keywordGene expression
dc.subject.otherAdult
dc.subject.otherBehavior
dc.subject.otherCircadian rhythm
dc.subject.otherDiurnal preference
dc.subject.otherFemale
dc.subject.otherGene
dc.subject.otherGene expression
dc.subject.otherHair follicle cell
dc.subject.otherHuman
dc.subject.otherHuman cell
dc.subject.otherMale
dc.subject.otherNight
dc.subject.otherNr1d2 gene
dc.subject.otherOscillation
dc.subject.otherPer3 gene
dc.subject.otherPolymerase chain reaction
dc.titleDiurnal preference predicts phase differences in expression of human peripheral circadian clock genes
dc.typeinfo:eu-repo/semantics/article
dc.version.typeinfo:eu-repo/semantics/publishedVersion
dc.volume.number13
dspace.entity.typePublication
person.identifier.scopus-author-id57191379504
person.identifier.scopus-author-id57191379650
person.identifier.scopus-author-id36807941500
person.identifier.scopus-author-id57190174014
person.identifier.scopus-author-id57191380656
person.identifier.scopus-author-id41861776800
person.identifier.scopus-author-id56323351500
person.identifier.scopus-author-id7005886727
relation.isAuthorOfPublication6f3f29e3-60b2-4801-9a85-612cddb42f4a
relation.isAuthorOfPublication.latestForDiscovery6f3f29e3-60b2-4801-9a85-612cddb42f4a
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