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Acetic acid stress response of the acidophilic sulfate reducer Acididesulfobacillus acetoxydans

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dc.contributor.authorEgas, Reinier
dc.contributor.authorSahonero Canavesi, Diana
dc.contributor.authorBale, Nicole
dc.contributor.authorKoenen, Micel
dc.contributor.authorYildiz, Çaglar
dc.contributor.authorVillanueva, Laura
dc.contributor.authorSousa, Diana
dc.contributor.authorSánchez Andrea, Irene
dc.contributor.funderMinisterie van Onderwijs, Cultuur en Wetenschap
dc.contributor.rorhttps://ror.org/02jjdwm75
dc.date.accessioned2024-07-08T13:14:59Z
dc.date.accessioned2024-09-16T11:20:38Z
dc.date.available2024-07-08T13:14:59Z
dc.date.available2024-09-16T11:20:38Z
dc.date.issued2024
dc.description.abstractAcid mine drainage (AMD) waters are a severe environmental threat,due to their high metal content and low pH (pH <3). Current technologies treating AMD utilize neutrophilic sulfate-reducing microorganisms (SRMs),but acidophilic SRM could offer advantages. As AMDs are low in organics these processes require electron donor addition,which is often incompletely oxidized into organic acids (e.g.,acetic acid). At low pH,acetic acid is undissociated and toxic to microorganisms. We investigated the stress response of the acetotrophic Acididesulfobacillus acetoxydans to acetic acid. A. acetoxydans was cultivated in bioreactors at pH 5.0 (optimum). For stress experiments,triplicate reactors were spiked until 7.5 mM of acetic acid and compared with (non-spiked) triplicate reactors for physiological,transcriptomic,and membrane lipid changes. After acetic acid spiking,the optical density initially dropped,followed by an adaptation phase during which growth resumed at a lower growth rate. Transcriptome analysis revealed a downregulation of genes involved in glutamate and aspartate synthesis following spiking. Membrane lipid analysis revealed a decrease in iso and anteiso fatty acid relative abundance; and an increase of acetyl-CoA as a fatty acid precursor. These adaptations allow A. acetoxydans to detoxify acetic acid,creating milder conditions for other microorganisms in AMD environments. © 2024 The Authors. Environmental Microbiology published by Applied Microbiology International and John Wiley & Sons Ltd.
dc.description.fundingtypeThis work was financed by the Soehngen Institute for Anaerobic Microbiology Gravitation Program (SIAM 024.002.002) a Gravitation Grant of the Netherlands Ministry of Education, Culture and Science. We thank Iame Alves Guedes and Ton van Gelder for their technical support. We thank Lot van der Graaf for her advice on the metabolism of A. acetoxydans and Daan van Vliet for his help with transcriptomics. This work was financed by the Soehngen Institute for Anaerobic Microbiology Gravitation Program (SIAM 024.002.002) a Gravitation Grant of the Netherlands Ministry of Education, Culture and Science. We thank Iame Alves Guedes and Ton van Gelder for their technical support. We thank Lot van der Graaf for her advice on the metabolism of and Daan van Vliet for his help with transcriptomics. A. acetoxydans
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dc.identifier.citationEgas, R. A., Sahonero Canavesi, D. X., Bale, N. J., Koenen, M., Yildiz, Ç., Villanueva, L., ... & Sánchez Andrea, I. (2024). Acetic acid stress response of the acidophilic sulfate reducer Acididesulfobacillus acetoxydans. Environmental Microbiology, 26(2), e16565.
dc.identifier.doihttps://doi.org/10.1111/1462-2920.16565
dc.identifier.issn14622912
dc.identifier.officialurlhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85185130712&doi=10.1111%2f1462-2920.16565&partnerID=40&md5=59d0df6252172713d7833a3127abf7ac
dc.identifier.urihttps://hdl.handle.net/20.500.14417/3277
dc.issue.number2
dc.journal.titleEnvironmental Microbiology
dc.language.isoeng
dc.page.total27
dc.publisherJohn Wiley and Sons Inc
dc.relation.departmentEnvironmental Sciences
dc.relation.entityIE University
dc.relation.projectIDSIAM 024.002.002
dc.relation.schoolIE School of Science & Technology
dc.rightsAttribution-NonCommercial 4.0 International
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subject.otheracetic acid
dc.subject.otheracetyl coenzyme A
dc.subject.otheraspartic acid
dc.subject.othercarboxylic acid
dc.subject.otherfatty acid
dc.subject.otherglutamic acid
dc.subject.othermembrane lipid
dc.subject.otherwater
dc.subject.otheracid mine drainage
dc.subject.otheracidity
dc.subject.otherarticle
dc.subject.otherbioreactor
dc.subject.othercontrolled study
dc.subject.otherdown regulation
dc.subject.othergene expression profiling
dc.subject.othergrowth rate
dc.subject.otherlipid analysis
dc.subject.othernonhuman
dc.subject.otheroptical density
dc.subject.otherpH
dc.subject.otherphysiological stress
dc.subject.othersulfate reducer
dc.subject.othersynthesis
dc.subject.otherAcetic Acid
dc.subject.otherAcids
dc.subject.otherBioreactors
dc.subject.otherFatty Acids
dc.subject.otherMembrane Lipids
dc.subject.otherSulfates
dc.titleAcetic acid stress response of the acidophilic sulfate reducer Acididesulfobacillus acetoxydans
dc.typeinfo:eu-repo/semantics/article
dc.version.typeinfo:eu-repo/semantics/publishedVersion
dc.volume.number26
dspace.entity.typePublication
relation.isAuthorOfPublicationf6e999b8-2aa8-4326-a4f3-d31ce5a41503
relation.isAuthorOfPublication.latestForDiscoveryf6e999b8-2aa8-4326-a4f3-d31ce5a41503
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