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A novel mechanism for dissimilatory nitrate reduction to ammonium in Acididesulfobacillus acetoxydans

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dc.contributor.authorEgas, Reinier
dc.contributor.authorKurth, Julia
dc.contributor.authorBoeren, Sjef
dc.contributor.authorSousa, Diana
dc.contributor.authorWelte, Cornelia
dc.contributor.authorSánchez Andrea, Irene
dc.contributor.funderMinisterie van Onderwijs, Cultuur en Wetenschap
dc.contributor.rorhttps://ror.org/02jjdwm75
dc.date.accessioned2024-07-08T13:14:59Z
dc.date.available2024-07-08T13:14:59Z
dc.date.issued2024
dc.description.abstractThe biological route of nitrate reduction has important implications for the bioavailability of nitrogen within ecosystems. Nitrate reduction via nitrite,either to ammonium (ammonification) or to nitrous oxide or dinitrogen (denitrification),determines whether nitrogen is retained within the system or lost as a gas. The acidophilic sulfate-reducing bacterium (aSRB) Acididesulfobacillus acetoxydans can perform dissimilatory nitrate reduction to ammonium (DNRA). While encoding a Nar-type nitrate reductase,A. acetoxydans lacks recognized nitrite reductase genes. In this study,A. acetoxydans was cultivated under conditions conducive to DNRA. During cultivations,we monitored the production of potential nitrogen intermediates (nitrate,nitrite,nitric oxide,hydroxylamine,and ammonium). Resting cell experiments were performed with nitrate,nitrite,and hydroxylamine to confirm their reduction to ammonium,and formed intermediates were tracked. To identify the enzymes involved in DNRA,comparative transcriptomics and proteomics were performed with A. acetoxydans growing under nitrate- and sulfate-reducing conditions. Nitrite is likely reduced to ammonia by the previously undescribed nitrite reductase activity of the NADH-linked sulfite reductase AsrABC,or by a putatively ferredoxin-dependent homolog of the nitrite reductase NirA (DEACI_1836),or both. We identified enzymes and intermediates not previously associated with DNRA and nitrosative stress in aSRB. This increases our knowledge about the metabolism of this type of bacteria and helps the interpretation of (meta)genome data from various ecosystems on their DNRA potential and the nitrogen cycle. © 2024 Egas et al.
dc.description.fundingtypeThis work was financed by the Soehngen Institute for Anaerobic Microbiology Gravitation Program (SIAM 024.002.002) a Gravitation grant of the Dutch Ministry of Education, Culture and Science. This work was by the Soehngen Institute for Anaerobic Microbiology Gravitation Program (SIAM 024.002.002) a Gravitation grant of the Dutch Ministry of Education, Culture and Science.
dc.description.keywordacid mine drainage
dc.description.keywordacidophilic sulfate-reducing bacteria
dc.description.keywordasrABC
dc.description.keywordDNRA
dc.description.keywordNirA
dc.description.keywordnitrite reduction
dc.description.keywordnitrosative stress
dc.description.keywordproteome
dc.description.keywordtranscriptome
dc.formatapplication/pdf
dc.identifier.citationEgas, R. A., Kurth, J. M., Boeren, S., Sousa, D. Z., Welte, C. U., & Sánchez-Andrea, I. (2024). A novel mechanism for dissimilatory nitrate reduction to ammonium in Acididesulfobacillus acetoxydans. Msystems, 9(3), e00967-23.
dc.identifier.doihttps://doi.org/10.1128/msystems.00967-23
dc.identifier.issn23795077
dc.identifier.officialurlhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85188128079&doi=10.1128%2fmsystems.00967-23&partnerID=40&md5=f0df2bc3ac1d46f787f26473607fc48e
dc.identifier.urihttps://hdl.handle.net/20.500.14417/3116
dc.issue.number3
dc.journal.titlemSystems
dc.language.isoeng
dc.page.total15
dc.publisherAmerican Society for Microbiology
dc.relation.departmentEnvironmental Sciences
dc.relation.entityIE University
dc.relation.projectIDOCW; SIAM 024.002.002
dc.relation.schoolIE School of Science & Technology
dc.rightsAttribution 4,0 International
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectacid mine drainage; acidophilic sulfate-reducing bacteria; asrABC; DNRA; NirA; nitrite reduction; nitrosative stress; proteome; transcriptome
dc.subject.keywordacid mine drainage
dc.subject.keywordacidophilic sulfate-reducing bacteria
dc.subject.keywordasrABC
dc.subject.keywordDNRA
dc.subject.keywordNirA
dc.subject.keywordnitrite reduction
dc.subject.keywordnitrosative stress
dc.subject.keywordproteome
dc.subject.keywordtranscriptome
dc.subject.otherammonia
dc.subject.otherbacterial enzyme
dc.subject.otherferredoxin
dc.subject.otherhydroxylamine
dc.subject.othermolybdopterin
dc.subject.othernitrate
dc.subject.othernitric oxide
dc.subject.othernitrite
dc.subject.othernitrite reductase
dc.subject.othernitrogen
dc.subject.othernitrous oxide
dc.subject.otheroxidoreductase
dc.subject.otherprotein AsrABC
dc.subject.otherprotein NirA
dc.subject.otherproteome
dc.subject.otherreduced nicotinamide adenine dinucleotide
dc.subject.othersulfate
dc.subject.othersulfite reductase
dc.subject.othertranscriptome
dc.subject.otherunclassified drug
dc.subject.otherammonium derivative
dc.subject.othernitric acid derivative
dc.subject.othernitrite reductase
dc.subject.othersulfate
dc.subject.otheracid mine drainage
dc.subject.otherAcididesulfobacillus acetoxydans
dc.subject.otherammonification
dc.subject.otherArticle
dc.subject.otherbacterial metabolism
dc.subject.otherbacterial strain
dc.subject.otherbioavailability
dc.subject.otherbiotransformation
dc.subject.othercell culture
dc.subject.othercomparative proteomics
dc.subject.othercontrolled study
dc.subject.otherdenitrification
dc.subject.otherDesulfosporosinus
dc.subject.otherDesulfosporosinus acididurans
dc.subject.otherDesulfosporosinus acidiphilus
dc.subject.otherdifferential gene expression
dc.subject.otherecosystem
dc.subject.otherenzyme activity
dc.subject.otherEscherichia coli
dc.subject.othergene expression level
dc.subject.othermetagenome
dc.subject.othernitrogen cycle
dc.subject.othernitrosative stress
dc.subject.othernonhuman
dc.subject.otherParacoccus denitrificans
dc.subject.otherreduction (chemistry)
dc.subject.othersediment
dc.subject.othersulfate reducing bacterium
dc.subject.otherthermodesulfobium narugense
dc.subject.othertranscriptomics
dc.subject.otherupregulation
dc.subject.otherbacterium
dc.subject.othermetabolism
dc.subject.otherAmmonium Compounds
dc.subject.otherBacteria
dc.subject.otherDenitrification
dc.subject.otherEcosystem
dc.subject.otherHydroxylamine
dc.subject.otherHydroxylamines
dc.subject.otherNitrates
dc.subject.otherNitrite Reductases
dc.subject.otherNitrites
dc.subject.otherNitrogen
dc.subject.otherSulfates
dc.titleA novel mechanism for dissimilatory nitrate reduction to ammonium in Acididesulfobacillus acetoxydans
dc.typeinfo:eu-repo/semantics/article
dc.version.typeinfo:eu-repo/semantics/publishedVersion
dc.volume.number9
dspace.entity.typePublication
person.identifier.scopus-author-id57206272824
person.identifier.scopus-author-id56924968700
person.identifier.scopus-author-id6603966715
person.identifier.scopus-author-id15926458300
person.identifier.scopus-author-id26424142700
person.identifier.scopus-author-id50263036400
relation.isAuthorOfPublicationf6e999b8-2aa8-4326-a4f3-d31ce5a41503
relation.isAuthorOfPublication.latestForDiscoveryf6e999b8-2aa8-4326-a4f3-d31ce5a41503
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